Towards multiplexed near-infrared cellular imaging using gold nanostar arrays with tunable fluorescence enhancement

Evaluation of Reflection Optical Imaging Characteristics Using Fluorescence with Near Infrared Wavelength at Different Depths of Tissue Equivalent Phantom

Multiplexed cytokine detection on plasmonic gold substrates with enhanced near-infrared fluorescence

Protein microarrays based on fluorescence detection have been widely utilized for high-throughput functional proteomic analysis. However, a drawback of such assays has been low sensitivity and narrow dynamic range, limiting their capabilities especially for detecting low abundance biological molecules such as cytokines in human samples. Here, we present fluorescence-enhanced microarrays by plas...

Near-Infrared Fluorescence Enhancement Using Silver Island Films

Near-infrared (near-IR) excitation produces little background signal from biological molecules, making nearIR fluorescence technology highly useful in proteomic and genomic applications. To increase the emissions of near-IR fluorophores, we examined the use of metal-enhanced fluorescence on these longer wavelength dyes. IRDye700and IRDye800-labeled DNA oligonucleotides and proteins were spotted...

Arthritis imaging using a near-infrared fluorescence folate-targeted probe

A recently developed near-infrared fluorescence-labeled folate probe (NIR2-folate) was tested for in vivo imaging of arthritis using a lipopolysaccharide intra-articular injection model and a KRN transgenic mice serum induction mouse model. In the lipopolysaccharide injection model, the fluorescence signal intensity of NIR2-folate (n = 12) and of free NIR2 (n = 5) was compared between lipopolys...

Fluorescence lifetime imaging microscopy using near-infrared contrast agents.

Although single-photon fluorescence lifetime imaging microscopy (FLIM) is widely used to image molecular processes using a wide range of excitation wavelengths, the captured emission of this technique is confined to the visible spectrum. Here, we explore the feasibility of utilizing near-infrared (NIR) fluorescent molecular probes with emission >700 nm for FLIM of live cells. The confocal micro...